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KMID : 0613820190290111192
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Journal of Life Science
2019 Volume.29 No. 11 p.1192 ~ p.1199
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Evaluation of Whitening Activity and Wrinkle Inhibitory Effect of Ethanol Extracts of Nelumbinis Rhizomatis Nodus
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Jang Young-Ah
Yeom Bo-Seul
Kim Se-Gie
Lee Jin-Tae
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Abstract
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The intention of this study was to confirm the possible use of an ethanol extracts??? of Nelumbinis Rhizomatis Nodus (NRN) as a cosmetic material??? Ingredient?. To this end, we extracted NRN with 70% ethanol and performed biological activity evaluation of whitening efficacy and wrinkle reduction. We performed cellular tyrosinase inhibition and melanin contents assay to check the whitening activity of NRN and carried out a toxicity evaluation of NRN via an MTT assay and the amounts of associated proteins that affect melanin production in a melanoma cell line (B16F10). And collagenase inhibitory assay was performed for the evaluation of anti-wrinkle of samples. In addition, a toxicity evaluation using an MTT assay and matrix metalloprotease (MMP-1) and procollagen synthesis inhibition by NRN were evaluated in a fibroblast cell line (CCD-986sk). Western blot results for the whitening activity evaluation revealed that the levels of two proteins related to melanin production, tyrosinase-related protein-1 (TRP-1) and tyrosinase-related protein-2 (TRP-2), were decreased in a dose-dependent manner. Moreover, collagenase inhibition activity at a concentration of 500 μg/ml NRN by measuring epigallocatechin-3-gallate (EGCG) was increased by more than 80% compared to the control group. Meanwhile, procollagen synthesis was reduced by 68.8% in the UVB-induced CCD- 986sk cells group whereas collagen synthesis recovered by 80.2% with 25 μg/ml NRN. The MMP-1 expression rate showed 20.2% reduction at 25 μg/ml. The results of the experiments verified the whitening and wrinkle suppression effects of NRN and confirmed that it could be used as a safe natural cosmetic material in the future.
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KEYWORD
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Cosmetic, Nelumbinis Rhizomatis Nodus, tyrosinase, whitening, wrinkle
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